The party said support from government body Scottish Enterprise has underpinned the growth in the sector.
In an answer to a parliamentary question submitted by SNP MSP Ash Denham, Fiona Hyslop said Scottish Enterprise had invested £11.5m in 38 video game companies over the five years since 2011.
Ms Denham said: "Scotland's video games sector has been booming, with just a handful of firms when the SNP came into office growing into more than a hundred now.
"This sector offers high quality jobs and exports around the world - with Rockstar North in particular a global brand.
"Support from Scottish Enterprise has underpinned the growth in this sector as part of our wider efforts to grow domestic businesses and increase exports - with latest figures showing Scottish exports rising faster than the rest of the UK.
"To build on that success, it's essential that Scotland remains in the world's largest single market after Brexit."
